Detection of mutation in 21 hydroxylase deficiency in patients with congenital adrenal hyperplasia by PCR
- Author:
Huu Kim Vo
- Publication Type:Journal Article
- Keywords:
gene mutation
- MeSH:
Mutation;
Adrenal Hyperplasia, Congenital;
Polymerase Chain Reaction
- From:Journal of Practical Medicine
2002;435(11):47-48
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
10 patients with congenital adrenal hydroxylase and 1 healthy person without disease participated to a study on the mutation of gene CYP210H (mutation in 21 hydroxylase deficiency) by PCR. Hair’s DNA released from nuclear by chelex 10%. The chain of DNA were identified well by PCR. The mutation in 21 hydroxylase deficiency caused inactivation of gene CYP 21 can be detected by PCR.
