Effects of Poly (ADP-ribose) Polymerase Inhibitor on Hypoxic-ischemic Injury in the Neonatal Rat Brain: 1H Magnetic Resonance Spectroscopic Study.
10.4097/kjae.2002.42.2.228
- Author:
Eun Ha SUK
1
;
Hyun Sook HWANG
;
Kun Ho LIM
;
Jung Hee LEE
;
Mi Jeung GWAK
;
Pyung Hwan PARK
Author Information
1. Department of Anesthesiology, Ulsan University College of Medicine, Seoul, Korea. phpark@www.amc.seoul.kr
- Publication Type:Original Article
- Keywords:
3, 4-Dihydro-5-[4-(1-piperidinyl) butoxy]-1 (2H)-isoquinolinone;
(1)H magnetic resonance spectroscopy;
hypoxic-ischemic brain injury
- MeSH:
Anesthesia;
Animals;
Apoptosis;
Aspartic Acid;
Brain Injuries;
Brain Ischemia;
Brain*;
Carotid Artery, Common;
Cerebrum;
Halothane;
Magnetic Resonance Spectroscopy;
Negotiating;
Neuroprotective Agents;
Oxygen;
Rats*;
Rats, Sprague-Dawley
- From:Korean Journal of Anesthesiology
2002;42(2):228-240
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Poly (ADP-ribose) polymerase (PARP) has been described as an important candidate for mediation of neurotoxicity after brain ischemia. This study was purposed to evaluate the effects of a PARP inhibitor on hypoxic-ischemic injury in the neonatal rat brain. In this study, a highly potent inhibitor of PARP, 3, 4-Dihydro-5-[4-(1-piperidinyl) butoxy]-1 (2H)-isoquinolinone (DPQ) was investigated. METHODS: Seven-day old Sprague-Dawley rat pups were used. The right common carotid artery was ligated under halothane anesthesia. After a recovery period of 3 hours, they were exposed to 8% oxygen at 37degreesC for about 120 minutes. The animals were divided into four groups: the pre-treatment group (n = 13) and post-treatment group (n = 21) were given DPQ 10 mg/kg and the pre-control group (n = 7) and post-control group (n = 14) were given a vehicle for controls. Pre-treatment and pre-control groups were injected 30 minutes prior to the hypoxic injury while post-treatment and post-control groups were injected 30 minutes after the hypoxic period intraperitoneally. The right cerebral hemisphere of the rats were examined with localized (1)H magnetic resonance spectroscopy on day 1 and 7 after the hypoxic insult. Lipid/N-acetyl aspartate (Lip/NAA) and lipid/creatine (Lip/Cr) ratios were used as apoptotic markers. On day 14, the degree of brain injury was scored by morphological changes. RESULTS: In the DPQ treated groups, the Lip/NAA and Lip/Cr ratios were lower than those of the control groups on day 1 after the hypoxic-ischemic injury (P < 0.05). However on day 7, only the ratios of the pre-treatment group were lower than those of the control group (P < 0.05). The degree of morphological changes of the brain injury on day 14 were lower in the DPQ treated groups (P < 0.05). CONCLUSIONS: These results suggest that DPQ exerts a neuroprotective effect in cerebral hypoxic-ischemic injury probably by inhibiting apoptosis especially in the early stage after an insult. Acute inhibition of PARP can have a therapeutic value in preventing ischemic brain injury.
