Tanshinone IIA, an ingredient of Salvia miltiorrhiza BUNGE, induces apoptosis in human leukemia cell lines through the activation of caspase-3.
- Author:
Hyun Jea SUNG
1
;
Sun Mi CHOI
;
Yoo Sik YOON
;
Kyu Suk AN
Author Information
1. Korea Institute of Oriental Medicine, Seoul. ysyoon66@hanmail.net
- Publication Type:Original Article
- Keywords:
apoptosis;
caspase-3;
leukemia;
Salvia miltiorrhiza BUNGE;
tanshinone II-A
- MeSH:
Antineoplastic Agents, Phytogenic/pharmacology*;
Antineoplastic Agents, Phytogenic/chemistry;
Apoptosis/physiology*;
Caspases/metabolism*;
Caspases/drug effects*;
Cell Cycle/drug effects;
DNA Fragmentation/drug effects;
Dose-Response Relationship, Drug;
Drugs, Chinese Herbal/pharmacology*;
Drugs, Chinese Herbal/chemistry;
Enzyme Activation/drug effects;
HL-60 Cells/pathology;
HL-60 Cells/metabolism;
HL-60 Cells/drug effects;
Human;
Lamiaceae/chemistry;
Leukemia/pathology*;
Leukemia/metabolism;
Leukemia/drug therapy;
Leukemia, Erythroblastic, Acute/pathology;
Leukemia, Erythroblastic, Acute/metabolism;
Leukemia, Erythroblastic, Acute/drug therapy;
Phenanthrenes/pharmacology*;
Phenanthrenes/chemistry;
Tumor Cells, Cultured
- From:Experimental & Molecular Medicine
1999;31(4):174-178
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tanshinone II-A is a derivative of phenanthrene-quinone isolated from Salvia miltiorrhiza BUNGE, a traditional herbal medicine that is known to induce antiinflammatory, anti-oxidative and cytotoxic activity. We have examined cellular effects of Tanshione II-A on HL60 human promyelocytic leukemic cells and K562 human erythroleukemic cells. Tanshione II-A induced a dose- and time-dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of poly(ADP-ribose) polymerase in both cell lines. PI-staining and flow cytometry analysis of K562 cells following Tanshione II-A treatment showed an increase of the cells possessing hypodiploid DNA indicative of apoptotic state of cells. Caspase-3 activity was significantly increased during Tanshinone II-A treatment of both HL60 and K562 cells, whereas caspase-1 activity was not changed. These results suggest that Tanshione II-A induced HL60 and K562 cellular apoptosis that may be associated with the selective members of caspase family. Copyright 2000 Academic Press.
