Culture and Polymerase Chain Reaction of Helicobacter pylori from Rectal and Terminal Ileal Fluid after Polyethylene Glycol (Colyte(R)) Ingestion in Healthy Adults with Positive Urea Breath Test.
- Author:
Do Hyun KIM
1
;
Hong Myong JUNG
;
Young Jun HWANG
;
Yong Soo AHN
;
Jang Sik MUN
;
Bo Hyun MYOUNG
;
Hyeuk PARK
;
Eun Joo JEONG
;
Yun Mi IM
;
Hyun Min OH
;
Hui Yeong JEONG
;
Chul PARK
;
Hyung Rag KIM
;
Eun Hae CHO
;
Ho Dong KIM
;
Young Do JUNG
Author Information
- Publication Type:Original Article ; English Abstract
- Keywords: Urea breath test; Helicobacter pylori; Polyethylene glycol; H. pylori DNA (flaA, 16S rRNA gene and cagA)
- MeSH: Adult; Antigens, Bacterial/genetics; Bacterial Proteins/genetics; Breath Tests; Electrolytes/administration & dosage; Feces/microbiology; Female; Helicobacter Infections/*diagnosis/transmission; Helicobacter pylori/genetics/*isolation & purification; Humans; Ileum/*microbiology; Male; Middle Aged; Polyethylene Glycols/administration & dosage; Polymerase Chain Reaction; RNA, Ribosomal, 16S/genetics; Rectum/*microbiology; Sensitivity and Specificity; Urea/analysis; Urease/genetics
- From:The Korean Journal of Gastroenterology 2010;56(1):27-32
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND/AIMS: Helicobacter pylori (H. pylori) transmission route is not yet clearly understood. Isolating H. pylori from stool, saliva, and vomitus is very difficult. However, H. pylori could be cultured from feces in the setting of rapid gastrointestinal tract transit. The aim of this study was to isolate H. pylori by culture and PCR in the rectum and terminal ileum during colonoscopy. METHODS: Twenty subjects with positive UBT (urea breath test) were included. We performed polymerase chain reaction (PCR) test and culture of H. pylori with the rectal fluid and terminal ileal fluid during colonoscopy. RESULTS: H. pylori was cultured with rectal fluid from 9 (45.0%) of 20 subjects and with ileal fluid from 11 (55.0%) of 20 subjects. H. pylori was a little more frequently cultured from the terminal ileal fluid than the rectal fluid without statistical significance (p>0.05). PCR test detected flaA (16/20, 80.0% and 17/20, 85.0%), 16S rRNA gene (16/20, 80.0% and 17/20, 85.0%), cagA (10/20, 50.0% and 12/20, 60.0%), and ureC (9/20, 45% and 11/20, 54.5%) from the rectal fluid and the terminal ileal fluid, respectively. The specificity and sensitivity of ureC were 100%. CONCLUSIONS: H. pylori could be cultured from the rectal fluid and terminal ileal fluid in the setting of rapid gastrointestinal tract transit. These results suggest of fecal-oral transmission of H. pylori.
