Production of Monoclonal Antibody to Chlamydia Pneumoniae.
- Author:
Tae Yeal CHOI
1
;
Jung Ok KANG
;
Deog Un KIM
;
Jeong Yeal AHN
;
Hyo Sun CHOI
Author Information
1. Department of Clinical Pathology, Hanyang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Chlamydia pneumoniae;
Monoclonal antibody;
Western blot
- MeSH:
Animals;
Antibodies;
Antibodies, Monoclonal;
Blotting, Western;
Chlamydia*;
Chlamydophila pneumoniae*;
Diagnosis;
Diatrizoate Meglumine;
Electrophoresis;
Enzyme-Linked Immunosorbent Assay;
Humans;
Mice;
Pneumonia;
Polyethylene Glycols;
Respiratory Tract Infections;
Sodium;
Spleen
- From:Korean Journal of Infectious Diseases
1997;29(2):139-146
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Chlamydia pneumoniae is a recently recognized species consisting of the strains commonly referred to as TWAR. These strains are associated with acute respiratory infections in humans, especially atypical pneumonia. So we tried to make a monoclonal antibody to Chlamydia pneumoniae. METHODS: C. pneumoniae were adapted to grow in HeLa-229 cells. The organisms were harvested and purified in a linear gradient of renograffin. BALB/c mice (female, 10weeks) were intravenously immunized with purified C. pneumoniae(TW-183).The spleen cells and SP 2/0 myeloma cells were fused with 40% polyethylene glycol (Mol.Wt.:1,450). Antibodies against C. pneumoniae were screened by an enzyme- linked immunosorbent assay (ELISA). The proteins of purified chlamydial elementary bodies were separated by discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western blots were performed with these monoclonal antibodies. RESULTS: Two monoclonal antibodies (HYMD10, HYMG12) reacted specifically with C. pneumoniae, as measured by an ELISA and indirect immunofluorecent stain. One of the monoclonal antibody (HYMD10) reacted with 75- and 39-KDa proteins in Western blot. The other monoclonal antibody (HYMG12) reacted with 98- and 39-KDa proteins of C. pneumoniae. CONCLUSIONS: These species-specific monoclonal antibodies (HYMD10, HYMG12) to C. pneumoniae could be used for diagnosis of C. pneumoniae infections.
