A Comparison Study of Metaphase Analysis of Chromosomal Aberration and Flow Cytometric Assessment of Radiation- induced Apoptosis in Human Peripheral Lymphocytes.
- Author:
Sang Ku LEE
;
Hee Seung BOM
;
Jung Jun MIN
;
Ho Cheon SONG
;
Hwan Jeong JEONG
;
Seung Yeon LEE
;
Ji Yeul KIM
;
Jong Hee SHIN
;
Sun Pal SUH
;
Dong Wook RHANG
- Publication Type:Original Article
- Keywords:
Metaphase;
Chromosome aberrations;
Flow cytometry;
Apoptosis;
Radiation injuries;
Lymphocytes
- MeSH:
Apoptosis*;
Chromosome Aberrations*;
Female;
Flow Cytometry;
Gamma Rays;
Healthy Volunteers;
Humans*;
Lymphocytes*;
Male;
Metaphase*;
Propidium;
Radiation Injuries;
Ring Chromosomes
- From:Korean Journal of Nuclear Medicine
1999;33(1):94-99
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Radiation-induced chromosomal damage and apoptosis were compared in human lymphocytes. MATERIALS AND METHODS: Peripheral lymphocytes from 10 normal volunteers (6 males, 4 females, age range 23~41 years) were irradiated by gamma rays from a cell irradiator. Doses of irradiation were 0 (control), 0.18, 2, 5, 10, 20 and 25 Gy. Irradiated lymphocytes were examined by metaphase analysis for chromosomal aberrations and by flow cytometry for apoptosis. RESULTS of both studies were compared according to dose. RESULTS: Number of dicentric and ring chromosomes (D+R) was 0.5+/-0.53 at baseline, which was significantly increased after radiation according to the dose. The fraction of cells showing annexin V-fluore-scein isothiocyanate uptake was 0.55+/-0.39%, which increased to 3.58+/-1.85% by 2 Gy irradiation, and then decreased. The fraction of cells showing propidium iodide (PI) uptake was 0.52+/-0.12%, which significantly increased according to dose (upto 15.64+/-5.99% by 20 Gy irradiation). D+R and PI uptake were well correlated (r=0.84, p<0.001). CONCLUSION: Radiation-induced chromosomal aberration was correlated to nuclear uptake of PI, a marker of late apoptosis.
