Rapid Diagnosis of Mycobacterium abscessus Bacteremia Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry.
- Author:
Eun Jeong WON
1
;
Yong Jun CHOI
;
Soo Hyun KIM
;
Jong Hee SHIN
Author Information
- Publication Type:Original Article
- Keywords: Bacteremia; MALDI-TOF; Mycobacterium Abscessus
- MeSH: Adrenal Insufficiency; Amikacin; Bacteremia*; Catheters; Cefoxitin; Central Venous Catheters; Clarithromycin; Delivery of Health Care; Diagnosis*; Female; Humans; Imipenem; Mass Spectrometry*; Middle Aged; Mycobacterium*
- From:Annals of Clinical Microbiology 2016;19(3):77-81
- CountryRepublic of Korea
- Language:English
- Abstract: Mycobacterium abscessus was isolated from cultures of seven blood samples from a 64-year-old diabetic female who was admitted due to steroid-unresponsive adrenal insufficiency. The isolates were difficult to identify using the conventional commercial systems, VITEK 2 (bioMérieux, France) or MicroScan (Siemens Healthcare Diagnostics, USA), but were rapidly identified as M. abscessus by a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based Bruker Biotyper system (Bruker Daltonics, USA). Identification of M. abscessus was confirmed by a reverse hybridizationbased assay (Genotype Mycobacterium CM/AS 12, Hain Lifescience) and direct sequencing of a heatshock protein gene. After removal of her central venous catheter, the patient was successfully treated with a combination therapy comprising clarithromycin, amikacin, cefoxitin, and imipenem. Our findings demonstrate that MALDI-TOF MS can facilitate rapid and accurate identification of M. abscessus from blood cultures, which enables prompt administration of appropriate therapy following catheter removal.
