Apoptosis and Expression of bcl-2, p53, and Ki-67 in Mycosis Fungoides.
- Author:
Jae Bong LEE
;
Ho Sun JANG
;
Chang Keun OH
;
Kyung Sool KWON
;
Jung Hum PARK
- Publication Type:Original Article
- Keywords:
Mycosis Fungoides;
bcl-2;
p53;
Ki-67;
Apoptosis
- MeSH:
Antibodies, Monoclonal;
Apoptosis*;
Carcinogenesis;
Cell Proliferation;
Dermatitis, Contact;
Developmental Biology;
Homeostasis;
Humans;
Ki-67 Antigen;
Lichen Planus;
Lymphoma, T-Cell, Cutaneous;
Mycosis Fungoides*;
Oncogenes
- From:Korean Journal of Dermatology
1999;37(5):603-609
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Mycosis fungoides(MF) is a form of cutaneous T cell lymphoma with clonal differentiation of helpr' T cell. It has a patch, plaque, and tumor stage. But pathogenetic factors controlling the development and progression of MF are still unclear. Apoptosis plays a major role in developmental biology and homeostasis. The bcl-2 oncogene prolongs ce11 life by inhibiting apoptosis. The mutant pS3 gene induces apoptosis indirectly. Ki-67 antigen is the cell proliferation marker. Recently, it has been shown that the relationships among them are important in the tumorigenesis of the various tumors. OBJECTIVE: The aim of this study was to examine the expression of these genes and apoptotic rate and clarify the relationship among them in the development and progression of MF. METHODS: The eighteen specimens from 8 patients with MF and 10 specimens from benign lymphocytic infiltrating diseases including 5 lichen planus, 3 lupus erythematosus, and 2 contact dermatitis were included. We performed immunoperoxidase staining(LSAB technique) using monoclonal antibodies including bc1-2, p~53, and Ki-67(MIB1). We used ApoptaqTM(Oncor) in situ labelling kit for detecting apoptotic cell.
