Analysis of Calcium-Binding Proteins of Mouse Spermatozoa by 2-Dimentional SDS-PAGE.
- Author:
Myung Chan GYE
1
Author Information
1. Department of Biology, Kyonggi University, Suwon, Korea. mcgye@kuic.kyonggi.ac.kr
- Publication Type:Original Article
- Keywords:
Calcium-binding proteins;
Spermatozoa;
Mouse
- MeSH:
Acrosome;
Acrosome Reaction;
Animals;
Calcimycin;
Calcium;
Calcium-Binding Proteins*;
Cell Membrane;
Databases, Protein;
Diagnosis;
Edetic Acid;
Electrophoresis, Polyacrylamide Gel*;
Infertility, Male;
Male;
Mice*;
Spermatozoa*
- From:Korean Journal of Andrology
2000;18(2):105-109
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To verify the expression of calcium-binding proteins in mouse spermatozoa. MATERIALS AND METHODS: Mouse sperm proteins were subjected to 2-dimentional SDS-PAGE combined with calcium shift in the presence or absence of Ca2+, Zn2+ or EDTA. RESULTS: In the epididymal sperm extracts, 10 kinds of protein spots showed mobility shift in the gel containing Ca2+. Most of the CBPs disappeared after the acrosome reaction (AR) induced by Ca2+-ionophore A23187, suggesting that they originated from acrosome and/or the plasma membrane overlaying the acrosome. CONCLUSIONS: The CBPs might be involved in acrosome reaction of mouse spermatozoa. Protein database of sperm CBPs might be useful for diagnosis of male infertility.
