Methylenetetrahydrofolate Reductase Polymorphisms and Risk of Recurrent Pregnancy Loss: a Case-Control Study.
10.3346/jkms.2017.32.12.2029
- Author:
Kyu Ri HWANG
1
;
Young Min CHOI
;
Jin Ju KIM
;
Sung Ki LEE
;
Kwang Moon YANG
;
Eun Chan PAIK
;
Hyeon Jeong JEONG
;
Jong Kwan JUN
;
Sang Ho YOON
;
Min A HONG
Author Information
1. Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. ymchoi@snu.ac.kr
- Publication Type:Original Article
- Keywords:
MTHFR Gene;
Recurrent Pregnancy Loss;
Single-Nucleotide Polymorphisms;
Korean
- MeSH:
Abortion, Habitual;
Abortion, Spontaneous;
Case-Control Studies*;
Female;
Fibrinolysis;
Genotype;
Humans;
Hyperhomocysteinemia;
Methylenetetrahydrofolate Reductase (NADPH2)*;
Platelet Aggregation;
Polymorphism, Single Nucleotide;
Pregnancy*;
Prospective Studies
- From:Journal of Korean Medical Science
2017;32(12):2029-2034
- CountryRepublic of Korea
- Language:English
-
Abstract:
The balance between coagulation and fibrinolysis is an essential part in early pregnancy. Mutations in methylenetetrahydrofolate reductase (MTHFR) gene lead to decreased activity of the enzyme and hyperhomocysteinemia, which then induces platelet aggregation by promoting endothelial oxidative damage, possibly resulting in adverse effect on maintenance of pregnancy. We investigated the role of MTHFR single nucleotide polymorphisms (SNPs), C677T and A1298C, in Korean patients with recurrent pregnancy loss (RPL). We conducted a prospective case-control study in the Korean population. Subjects included 302 women with 2 or more consecutive, unexplained, spontaneous miscarriages before 20 weeks of gestation and 315 control women without a history of recurrent miscarriages. The genotyping for C677T and A1298C polymorphisms was performed using the TaqMan assay. Continuous variables were compared using Student's t-test, and χ² test was used to evaluate differences in the genotype distributions between the RPL and the controls. The genotype distribution of both polymorphisms in the RPL group did not differ from those of the controls. For further analysis, if RPL patients were divided according to the numbers of pregnancy losses (≥ 2 and ≥ 3) neither group was significantly different compared with controls. MTHFR gene C677T and A1298C polymorphisms are not associated with idiopathic RPL in Korean women, suggesting that those may not be susceptible allelic variants or be deficient to cause RPL.
