A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study.
10.5395/rde.2013.38.4.204
- Author:
Gautam Pyarelal BADOLE
1
;
Manjusha Madhukar WARHADPANDE
;
Ganesh Kothiramji MESHRAM
;
Rakesh Namdeoraoji BAHADURE
;
Shubha Gopal TAWANI
;
Gopal TAWANI
;
Shital Gautam BADOLE
Author Information
1. Department of Conservative Dentistry & Endodontics VSPM's Dental College & Research Center, Nagpur, India. badole_g15@yahoo.co.in
- Publication Type:In Vitro ; Original Article
- Keywords:
Cell culture;
Cytotoxicity;
MTT Assay;
Root canal sealers
- MeSH:
Animals;
Bismuth;
Calcium Hydroxide;
Cell Culture Techniques;
Cell Line;
Dental Pulp Cavity*;
Dexamethasone;
Drug Combinations;
Epoxy Resins;
Fibroblasts;
Formaldehyde;
Hydrocortisone;
Incubators;
Mice;
Polytetrafluoroethylene;
Root Canal Filling Materials;
Silver;
Thymol;
Titanium
- From:Restorative Dentistry & Endodontics
2013;38(4):204-209
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929). MATERIALS AND METHODS: Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis. RESULTS: Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity. CONCLUSIONS: Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.
