Additive Effects of Intra-articular Injection of Growth Hormone and Hyaluronic Acid in Rabbit Model of Collagenase-induced Osteoarthritis.
10.3346/jkms.2010.25.5.776
- Author:
Sang Beom KIM
1
;
Dong Rak KWON
;
Hyun KWAK
;
Yong Beom SHIN
;
Hyun jung HAN
;
Jong Hwa LEE
;
Seok Hwa CHOI
Author Information
1. Department of Rehabilitation Medicine, Arthritis & Autoimmunity Research Center, Catholic University of Daegu School of Medicine, Daegu, Korea. coolkwon@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Osteoarthritis;
Hyaluronic Acid;
Growth Hormone;
Collagenases
- MeSH:
Animals;
*Collagenases;
*Disease Models, Animal;
Drug Combinations;
Drug Synergism;
Human Growth Hormone/*administration & dosage;
Humans;
Hyaluronic Acid/*administration & dosage;
Injections, Intra-Articular;
Male;
Osteoarthritis/*chemically induced/diagnosis/*drug therapy;
Rabbits;
Treatment Outcome
- From:Journal of Korean Medical Science
2010;25(5):776-780
- CountryRepublic of Korea
- Language:English
-
Abstract:
In a rabbit model of collagenase-induced osteoarthritis, the additive effects of intra-articular recombinant human growth hormone (GH) administration to hyaluronic acid (HA) were evaluated. After intra-articular collagenase injection, mature New Zealand white rabbits (n=30) were divided into 3 groups. Group 1 (control rabbits) received once weekly intra-articular saline injections for 4 weeks. Group 2 rabbits received 6 mg HA injections, and group 3 rabbits were injected with 6 mg HA and 3 mg recombinant human GH. These injections were initiated 4 weeks after collagenase injections. Lameness was observed for 9 weeks after collagenase injections. Macroscopic and histopathological knee joint findings were also evaluated at the end of 9 weeks after collagenase injections. Although all animals had lameness after collagenase injections, the duration and severity of lameness were significantly shorter and less severe in group 3 than group 1 and 2 (P<0.01). Macroscopic scores showed that femoral condyles of group 3 rabbits received significantly less cartilage damage than those of groups 1 and 2 rabbits (P<0.01). Histopathological score was also the lowest in group 3 (P<0.01). These results suggest that co-injection of intra-articular HA and recombinant human GH is more effective than HA injections alone in an osteoarthritis model.
