Sulfidoleukotrienes Production in Blood Leukocytes Stimulated by Mite Allergen.
- Author:
Man Yong HAN
1
;
Kwang Eun CHA
;
Seung Yeon NAM
;
Kwang Shin LEE
;
Young Jae KOH
;
Kang Mo AHN
;
Sang Il LEE
Author Information
1. Department of Pediatrics, Pundang CHA General Hospital, College of Medicine, Pochon CHA University, Sungnam, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Allergy;
Sulfidoleukotriens;
Cell stimulation
- MeSH:
Asthma;
Child;
Chronic Disease;
Dermatophagoides farinae;
Dermatophagoides pteronyssinus;
Diagnosis;
Eosinophils;
Humans;
Hypersensitivity;
Immunoglobulin E;
Infant;
Leukocytes*;
Mites*;
Skin
- From:Pediatric Allergy and Respiratory Disease
1999;9(4):375-384
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Although asthma is the most common chronic disease in childhood, accurate diagnosis in infants and young children remains challenging clinicians. Allergen challenging tests in vitro have been used productively as an investigative tool in studies of the pathophysiology and diagnosis of asthma. Therefore, we compared the sulfidoleukotrien (sLT) concentration according to allergen leukocyte stimulation test, in normal versus asthmatic patients, to find better diagnostic tools. METHODS: From May through August, 1998, nine children were enrolled who presented positive skin reaction in Dermatophagoides pteronyssinus (D.p.), Dermatophagoides farinae (D.f.) as patient groups. We measured total eosinophil count, serum IgE, sLT concentration of three different allergen stimulation (100 ng/ml, 10 ng/ml, 1 ng/ml). RESULTS: sLT concentration in three different D.p., D.f. stimulation showed significant differences (P<0.01). Allergen concentration of 10 ng/ml was fit for stimulating peripheral leukocyte. The sLT concentration is correlated with IgE, total eosinophil count, but not with age. Actual concentrations of sLT was not measured in allergen stimulation test. Its interpretation of test results was complicated by the fact that several variants were involved in determining sampling time and appropriate sampling volume. Most importantly, the diagnostic sensitivity of the sLT concentration tests varies directly with the magnitude of IgE antibody and total eosinophil count. CONCLUSION: We emphasizes the role of allergen challenge in understanding the pathophysiology of young children asthma. It focuses on more accurate diagnosis with objective techniques for analyzing the leukocyte sLT release to antigen.
