Evaluation of Arterial Impairment after Experimental Gelatin Sponge Embolization in a Rabbit Renal Model.
10.3348/kjr.2015.16.1.133
- Author:
Jung Suk OH
1
;
Hae Giu LEE
;
Ho Jong CHUN
;
Byung Gil CHOI
;
Yeong Jin CHOI
Author Information
1. Department of Radiology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. hgleehfh@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Gelatin sponge;
Absorbable;
Embolization;
Arterial stenosis
- MeSH:
Animals;
Constriction, Pathologic/*etiology;
Disease Models, Animal;
Embolization, Therapeutic/*adverse effects;
Gelatin;
Gelatin Sponge, Absorbable/*chemistry;
Kidney/*blood supply;
Male;
Porifera;
Rabbits;
Renal Artery/*pathology/radiography;
Swine
- From:Korean Journal of Radiology
2015;16(1):133-138
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Arterial stenosis is a major obstacle for subsequent interventional procedures. We hypothesized that the stenosis is caused by gelatin sponge embolization and performed an experimental study in a rabbit renal model. MATERIALS AND METHODS: A total of 24 rabbits were embolized with porcine gelatin sponge particles injected into the renal arteries. Four rabbits were sacrificed on 1 day, 4 days, 1 week, 2 weeks, 3 weeks, and 4 weeks after embolization. Microscopic evaluations were performed on hematoxylin-eosin and smooth muscle actin immunohistochemical stained sections. RESULTS: Gelatin sponge particles were mainly observed in the segmental and interlobar arteries. Transmural inflammation of the embolized arterial wall and mild thickening of the media were observed 1 week after embolization. Resorption of the gelatin sponge and organization of thrombus accompanied by foreign body reactions, were observed from 2 to 4 weeks after embolization. Microscopic images of the 3 weeks group showed vessel lumens filled mostly with organized thrombi, resulting in severe stenosis. Additionally, vessels showed a thickened intima that contained migrating smooth muscle cells and accompanying interruption of the internal elastic lamina. The migrating smooth muscle cells were distributed around the recanalized arterial lumen. CONCLUSION: Gelatin sponge embolization may induce arterial stenosis by causing organized thrombus and intimal hyperplasia, which consists of migrating smooth muscle cells and intimal collagen deposits.
