A Role of gamma-subunit in FcgammaRII Signal Transduction of Platelets.
- Author:
Hwang Min KIM
;
Baek Keun LIM
- Publication Type:Original Article
- Keywords:
Platelet;
FcgammaRII receptor;
gamma-subunit
- MeSH:
Animals;
Antiphospholipid Syndrome;
Blood Platelets;
Collodion;
Edetic Acid;
Electrophoresis;
Heparin;
Immunoprecipitation;
Membranes;
Mice;
Platelet Activation;
Receptors, Fc;
Signal Transduction*
- From:Korean Journal of Pediatric Hematology-Oncology
1997;4(1):48-54
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The FcgammaRII receptor, the only kind of Fc receptor in the platelet, plays important role for immune-complex mediated platelet activation, which occurs in Heparin induced thrombocytopenia(HIT) or antiphospholipid syndrome. The purpose of this research is to find out the role of gamma-subunit in FcgammaRII signal transduction of platelets. METHODS: After cross-linking the FcgammaRII receptors with IV.3 anti FcgammaRII specific antibody and Rabbit anti mouse antibody, we made the platelet lysate in the RIPA EDTA lysis buffer condition, and performed anti gamma-subunit immunoprecipitation. After polyacylamide gel electrophoresis and Western transfer, we probed the nitrocellulose membrane of gamma-subunit immunoprecipitate with anti gamma-subunit antibody. RESULTS: The mobility shift of gamma-subunit was observed upon FcgammaRII cross-linking. CONCLUSION: The gamma-subunit is activated upon FcgammaRII cross-linking, which means the gamma-subunit is involved in FcgammaRII signal transduction of platelet.
