Induction of CEA-specific Cytotoxic T Lymphocytes by Murine Dendritic Cells Expressing CEA.
- Author:
Eun Ha WON
1
;
Chang Hyun KIM
;
Mi Young PARK
;
Hyun Il CHO
;
Seong Taek OH
;
Yong Kil HONG
;
Tai Gyu KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Carcinoembryonic antigen (CEA); adenovirus; dendritic cell (DC); cytotoxic T lymphocytes (CTL)
- MeSH: Adenoviridae; Animals; Bone Marrow; Carcinoembryonic Antigen; Dendritic Cells*; Enzyme-Linked Immunospot Assay; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Immunotherapy; Interleukin-4; Mice; T-Lymphocytes; T-Lymphocytes, Cytotoxic*; Vaccination
- From:Immune Network 2003;3(4):295-301
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Carcinoembryonic antigen (CEA) is well-known soluble tumor marker frequently detectable in peripheral blood of carcinoma patients and considered as good target for antigen-specific immunotherapy. In this study, we used a replication-deficient adenovirus containing CEA to study CTL induction in vitro after adenovirus-mediated gene transfer into DC. METHODS: DC were obtained from mouse bone marrow and cultured with IL-4 and GM-CSF. For measuring CTL activity, splenocytes were harvested from the mice, which were immunized with DC that had been infected AdV-CEA or pulsed with CEA peptide. Untreated DC was used as a control. Splenocytes were re-stimulated in vitro with DC pulsed with CEA peptide for 7 days and CTL activity with CEA peptide-pulsed EL-4 cells were assessed in a standard 51Cr-release assay. The frequencies of antigen-specific cytokine-secreting T cell were determined with mIFN-gamma ELISPOT. RESULTS: DC infected with recombinant adenovirus expressing CEA induced CEA-specific CTL responses in vivo. Splenocyte induced from mice immunized with AdV-CEA-infected DC increase in the number of IFN-gamma secreting T cells compared with those from mice immunized with CEA peptide-pulsed DC. CONCLUSION: These results suggested that DC infected with recombinant adenovirus has advantages over other forms of vaccination and could provide an alternative approach vaccination therapies.
