Inhibition of Fas-mediated Apoptosis of Rheumatoid Synovial Cells by TGF-beta and IL-1 beta.
- Author:
Seong Ho KIM
1
;
Won Chun BYUN
;
Mi Kyung LIM
;
You Sook CHO
;
Chan KIM
;
Mi Jung KIM
;
Bin YOO
;
Hee Bom MOON
Author Information
1. Department of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Rheumatoid synovium;
Apoptosis;
TFG-beta;
IL-1beta
- MeSH:
Apoptosis*;
Arthritis, Rheumatoid;
Cell Proliferation;
Flow Cytometry;
Interleukin-1*;
Interleukin-1beta*;
Joints;
Transforming Growth Factor beta*
- From:The Journal of the Korean Rheumatism Association
1999;6(2):110-116
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the proliferative and anti-apoptotic effects of TGF-beta and IL-1beta on rheumatoid synovial cells. METHODS: Synovial cells obtained from surgical procedure of the rheumatoid joint were cultured with TFG-beta and IL-1beta The proliferative response of synovial cells was examined by non-radioactive cell proliferation assay. Fas-demiated apoptosis of synovial cells was measured by flow cytometry after addition of anti-Fas antibody. RESULTS: TGF-beta and IL-1betaproliferated synovial cells in a dose-dependent manner. They also made synovial cells resistant to anti-Fas antibody induced apoptosis. CONCLUSION: TGF-beta and IL-1betapromotes synovial cell proliferation possibly through interference with Fas-mediated apoptosis, suggesting their role in synovial hyperplasis in rheumatoid arthritis.
