Cellular responses on anodized titanium discs coated with 1 alpha,25-dihydroxyvitamin D3 incorporated Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles.
10.4047/jkap.2008.46.6.620
- Author:
Young Jin CHO
1
;
Seong Joo HEO
;
Jai Young KOAK
;
Seong Kyun KIM
;
Joo Hee LEE
Author Information
1. Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University, Korea. ksy0617@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
1 alpha;
25-dihydroxyvitamin D3;
PLGA;
Nanoparticle;
Anodized titanium;
Cell proliferation;
Differentiation
- MeSH:
Calcitriol;
Cell Proliferation;
Cytoplasm;
Humans;
Lactic Acid;
Nanoparticles;
Osteosarcoma;
Polyglycolic Acid;
Pseudopodia;
Seeds;
Titanium;
Vitamin D
- From:The Journal of Korean Academy of Prosthodontics
2008;46(6):620-627
- CountryRepublic of Korea
- Language:English
-
Abstract:
STATEMENT OF PROBLEM: A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces. PURPOSE: The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/1alpha, 25-(OH)2D3 coating with reference to cellular proliferation and differentiation in vitro. MATERIAL AND METHODS: 96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/1alpha, 25-(OH)2D3 solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out. RESULTS: PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated 1 alpha, 25-(OH)2D3 (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05). CONCLUSION: Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface.
