IL-6 gene promoter polymorphisms in Korean generalized aggressive periodontitis.
10.5051/jkape.2008.38.4.579
- Author:
Sun Jung BANG
1
;
Il Shin KIM
;
Ok Su KIM
;
Young Jun KIM
;
Hyun Ju CHUNG
Author Information
1. Department of Periodontology, School of Dentistry, and Dental Science Research Institute, Korea. hjchung@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
aggressive periodontitis;
IL-6 gene;
SNP;
gene polymorphism
- MeSH:
Aggressive Periodontitis;
Alleles;
Cohort Studies;
Digestion;
DNA;
Female;
Genotype;
Humans;
Interleukin-6;
Male
- From:The Journal of the Korean Academy of Periodontology
2008;38(4):579-588
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to investigate the association of generalized aggressive periodontitis with IL-6 promoter gene single nucleotide polymorphisms(SNP). MATERIAL AND METHODS: The study population consisted of 52 generalized aggressive periodontitis patients(GAP) and 30 periodontally healthy control subjects, who were systemically healthy non-smokers. Genomic DNA was obtained from buccal swab. The IL-6 promotor SNP at the positions of -597, -572, and -174 were genotyped by amplifying the polymorphic region using polymerase chain reaction(PCR), restriction enzyme digestion and gel electrophoresis. RESULT: The genotype distributions for G/G, G/A and A/A genotypes of IL-6 -597 were 30.8%, 40.4%, and 28.8% in the GAP group and 53.3%, 40%, and 6.7% in the control group and were statistically different between 2 groups(p<0.05). Allele 2 frequency of IL-6 -597 were significantly higher in the GAP group than the control group(p<0.01). At the position of IL-6 -572, the distribution for C/C, C/G and G/G genotypes were 23.1%, 55.8% and 21.2% in the GAP group and 20%, 33.3%, and 46.7% in the control group. In female subjects, the genotype distribution were significantly different between 2 groups (p<0.01). In male subjects, allele 2 frequency of IL-6-572 was significantly lower in the GAP group than the control group(p<0.05). The genotype distribution of IL-6 -174 in the GAP group were 96.2%, 3.8% for G/G, G/C genotypes whereas only the G/G genotype was detected in the control group. CONCLUSION: In conclusion, significant associations were found in IL-6 gene promoter(-597, -572) polymorphisms and generalized aggressive periodontitis. Further cohort study will be necessary in larger population.
