Diagnosis of Tuberculous Meningitis by Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) in CSF.
- Author:
Won Joo KIM
1
;
Yoon Joong KIM
;
Soo Chul PARK
;
Byung In LEE
Author Information
1. Department of Neurology, College of Medicine, Yonsei University.
- Publication Type:Original Article
- MeSH:
Cerebrospinal Fluid;
Diagnosis*;
Early Diagnosis;
Enzyme-Linked Immunosorbent Assay*;
Humans;
Immunoglobulin G;
Polymerase Chain Reaction*;
Sensitivity and Specificity;
Tuberculin;
Tuberculosis, Meningeal*
- From:Journal of the Korean Neurological Association
1992;10(1):1-7
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Cerebrospinal fluid from 27 patients with tuberculous meningitis and from appropriate control individuals were assayed for immunoglobulin G activity to tuberculin purified protein derivative (PPD) and Lipoarabinomannan-B (LAM-B) antigens by a'n enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). A significant difference was found between the tuberculous meningitis group and control. The sensitivity and specificity of these tests in cerebrospinal fluid was 59.2% and 93.9% for PPD, 85.2% and 95.9% for LAM-B antigen, and 70.3% and 89.7% for PCR method. The ELISA method measuring IgG antibody to LAM-B in cerebrospinal fluid and PCR method were both reliable and complementary each other for early diagnosis of patient with tuberculous meningitis.
