Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells.
- Author:
Da In CHOI
1
;
Jee Young CHOI
;
Young Jee KIM
;
Jee Bum LEE
;
Sun Ouck KIM
;
Hyong Taek SHIN
;
Seung Chul LEE
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Anti-inflammatory activity; Anti-oxidant activity; p-Methoxy-N-methylphenethylamine; Ethanol extract of peanut sprouts; Oxazolone-induced contact dermatitis
- MeSH: Animals; Biomarkers; Cyclooxygenase 2; Dermatitis, Contact*; Ethanol*; Flow Cytometry; Inflammation; Mice*; Nerve Growth Factor; NF-kappa B; Oxazolone; p-Methoxy-N-methylphenethylamine; Reactive Oxygen Species; RNA; Skin
- From:Annals of Dermatology 2015;27(2):142-151
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: We developed an ethanol extract of peanut sprouts (EPS), a peanut sprout-derived natural product, which contains a high level of trans-resveratrol (176.75 microg/ml) and was shown to have potent antioxidant activity. OBJECTIVE: We evaluated the potential anti-inflammatory activity of EPS by measuring its antioxidant potential in skin. METHODS: The anti-inflammatory activity of EPS was tested using two models of skin inflammation: oxazolone (OX)-induced contact dermatitis in mice and compound 48/80-treated HaCaT cells. As biomarkers of skin inflammation, cyclooxygenase-2 (COX-2) and nerve growth factor (NGF) levels were measured. RESULTS: OX-induced contact dermatitis was suppressed markedly in mice that were treated with an ointment containing 5% EPS as evidenced by a decrease in the extent of scaling and thickening (p<0.05) and supported by a histological study. COX-2 (messenger RNA [mRNA] and protein) and NGF (mRNA) levels, which were upregulated in the skin of OX-treated mice, were suppressed markedly in the skin of OX+EPS-treated mice. Consistent with this, compound 48/80-induced expression of COX-2 (mRNA and protein) and NGF (mRNA) in HaCaT cells were suppressed by EPS treatment in a dose-dependent manner. As an inhibitor of NF-kappaB, IkappaB protein levels were dose-dependently upregulated by EPS. Fluorescence-activated cell sorting (FACS) analysis revealed that EPS scavenged compound 48/80-induced reactive oxygen species (ROS) in HaCaT cells. CONCLUSION: EPS exerts a potent anti-inflammatory activity via its anti-oxidant activity in both mouse skin and compound 48/80-treated HaCaT cells in vitro. Compound 48/80-treated HaCaT cells are a useful new in vitro model of skin inflammation.
