Investigation of Oxidative Stress and Development of Lower Urinary Tract Symptoms in the Urinary Bladder following Partial Bladder Outlet Obstruction by Proteomic Approach.
- Author:
Jung Hyun SHIM
1
;
Hyung Jee KIM
Author Information
1. Department of Urology, Dankook University College of Medicine, Cheonan, Korea. killtumor@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Bladder outlet obstruction;
Proteomics;
Oxidative stress
- MeSH:
Acetylcholine;
Animals;
Down-Regulation;
Electrophoresis, Gel, Two-Dimensional;
Humans;
Lower Urinary Tract Symptoms*;
Mass Spectrometry;
Oxidative Stress*;
Peroxiredoxins;
Proteomics;
Rats;
Thioredoxins;
Up-Regulation;
Urinary Bladder Neck Obstruction*;
Urinary Bladder*;
Urodynamics
- From:Korean Journal of Urology
2005;46(12):1337-1343
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study investigated the pathophysiological mechanism using the proteomic approach to detect the marker proteins for the development of lower urinary tract symptoms following a partial bladder outlet obstruction (BOO). MATERIALS AND METHODS: Rats were randomized into 3 groups, the control, sham operation and BOO groups. The BOO group was divided into 1, 3, and 5 day-groups. Conventional proteomics was performed using high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry using the rat urinary bladders. RESULTS: A comparison of the bladder from the BOO group with that from the sham control bladder showed three proteins, optineurin (OPTN), thioredoxin and preprohaptoglobin, to be over-expressed in the bladder of the BOO group. In addition, four proteins; peroxiredoxin 2, transgelin, hippocampal cholinergic neurostimulating peptide (HCNP) and beta-galactoside-binding lectin were under-expressed in the bladder of the BOO group. CONCLUSIONS: The down-regulation of HCNP might make the detrusor muscle supersensitive to acetylcholine, and the up-regulation of OPTN indicates protection from nerve injury. In addition, the up-regulation of thioredoxin and preprohaptoglobin and the down-regulation of peroxiredoxin 2 indicate that BOO may be related to oxidative stress. However more information on human bladder tissue will be needed for clinical usage and a urodynamic study.
