Plasmid Profile and B - Lactamase Type of Multidrug - Resistant Salmonella typhi Isolated from Korea, 1997.
- Author:
Jung Sik YOO
;
Young Hack SHIN
;
Kyung Soo OH
;
Jeom Kyu LEE
;
Ki Sang KIM
- Publication Type:Original Article
- Keywords:
Salmonella typhi;
Multidrug-resistant;
Plasmid;
B-lactamase
- MeSH:
Amoxicillin;
Asia, Southeastern;
Chloramphenicol;
Deoxyribonuclease HindIII;
Gyeonggi-do;
Humans;
Isoelectric Focusing;
Korea*;
Plasmids*;
Polymerase Chain Reaction;
Salmonella typhi*;
Salmonella*;
Tetracycline;
Tissue Donors;
Trimethoprim
- From:Journal of the Korean Society for Microbiology
1999;34(5):445-452
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Eight strains of multidrug-resistant (MDR) Salmonella typhi were isolated from Kyonggi area during January-February,1997. They were resistant to ampiciUin, amoxicillin, carbeniciillin, tetracycline, chloramphenicol, trimethoprim/sulfamethoxazole, trimethoprim. Eight strains had one plasmid respectively which size was approximately M.W 220 kb and showed same restriction pattern by endonuclease HindIII. The plasmid was similar to the plasmid in size that was related to multidrug resistant S. typhi isolated from southeast Asia. It were transferred by conjugation to recipient E, coli K-12 in frequency of 2.43 x10-4 - 1.73 x 10-2 and transconjugant showed same drug-resistant pattem with donor cells. All of 8 strains produced B-lactamase that was assummed to TEM-1 type by isoelectric focusing and PCR.
