Oxidation of fatty acid may be enhanced by a combination of pomegranate fruit phytochemicals and acetic acid in HepG2 cells.
- Author:
Ji Yeon KIM
1
;
Elly OK
;
You Jin KIM
;
Kyoung Sook CHOI
;
Oran KWON
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Vinegar beverage; acetic acid; pomegranate; fatty acid oxidation; HepG2 cells
- MeSH: Acetic Acid; Acyl-CoA Oxidase; Beverages; Blueberry Plant; Body Weight; Chromatography, Reverse-Phase; Drinking; Ellagic Acid; Fruit; Gene Expression; Hep G2 Cells; Hepatocytes; Humans; Mass Spectrometry; Morus; Opuntia; Overweight; Panax; PPAR alpha; Prunus; Punicaceae; RNA, Messenger
- From:Nutrition Research and Practice 2013;7(3):153-159
- CountryRepublic of Korea
- Language:English
- Abstract: We investigated whether the combination of phytochemicals and acetic acid in the form of fruit vinegar provides an additive effect on changes of mRNA levels related to fatty acid oxidation in human hepatocyte (HepG2). Among the seven fruit vinegars (Rubuscoreanus, Opuntia, blueberry, cherry, red ginseng, mulberry, and pomegranate) studied, treatment of HepG2 with pomegranate vinegar (PV) at concentrations containing 1 mM acetic acid showed the highest in vitro potentiating effect on the mRNA expression levels of peroxisome proliferator-activated receptor alpha, carnitinepalmitoyl transferase-1, and acyl-CoA oxidase compared to the control group (P < 0.05). Reversed-phase liquid chromatography in combination with quadrupole time-of-flight mass spectrometry analysis revealed four potential compounds (punicalagin B, ellagic acid, and two unidentified compounds) responsible for altered gene expression in HepG2 cells treated with PV as compared with the others. Further investigations are warranted to determine if drinking PV beverages may help to maintain a healthy body weight in overweight subjects.
