Peanut sprout ethanol extract inhibits the adipocyte proliferation, differentiation, and matrix metalloproteinases activities in mouse fibroblast 3T3-L1 preadipocytes.
- Author:
Woo Kyoung KIM
1
;
Nam E KANG
;
Myung Hwan KIM
;
Ae Wha HA
Author Information
- Publication Type:Original Article
- Keywords: Peanut sprout extract; resveratrol; adipocytes; matrix metalloproteinases; glycerol-3-phosphate dehydrogenase
- MeSH: 3T3-L1 Cells; Adipocytes; Animals; Cell Proliferation; Ethanol; Fibroblasts; Glycerolphosphate Dehydrogenase; Matrix Metalloproteinases; Mice; RNA, Messenger; Stilbenes
- From:Nutrition Research and Practice 2013;7(3):160-165
- CountryRepublic of Korea
- Language:English
- Abstract: 3T3-L1 preadipocyte were differentiated to adipocytes, and then treated with 0, 10, 20, and 40 microg/mL of peanut sprout ethanol extract (PSEE). The main component of PSEE is resveratrol which contained 5.55 mg/mL of resveratrol. The MTT assay, Oil-Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, and the triglyceride concentration were determined in 3T3-L1 cells. MMP-2 and MMP-9 activities as well as mRNA expressions of C/EBP beta and C/EBP alpha were also investigated. As the concentration of PSEE in adipocytes increased, the cell proliferation was decreased in a dose-dependent manner from 4 days of incubation (P < 0.05). The GDPH activity (P < 0.05) and the triglyceride concentration (P < 0.05) were decreased as the PSEE treatment concentration increased. The mRNA expression of C/EBPbeta in 3T3-L1 cells was significantly low in groups of PSEE-treated, compared with control group (P < 0.05). The MMP-9 (P < 0.05) and MMP-2 (P < 0.05) activities were decreased in a dose-dependent manner as the PSEE concentration increased from 20 microg/mL. In conclusion, it was found that PSEE has an effect on restricting proliferation and differentiation of adipocytes.
