The pH-dependent effects of combining ethanol with fluoride on proton permeability in Streptococcus mutans.
10.11149/jkaoh.2016.40.4.255
- Author:
Sae A LEE
1
;
Seung Il JUNG
;
Jin Bom KIM
;
Jung Sook KANG
Author Information
1. Department of Oral Biochemistry & Molecular Biology, School of Dentistry, Pusan National University, Yangsan, Korea. jsokang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
ethanol;
F-ATPase;
Fluoride;
pH stress;
proton permeability;
Streptococcus mutans
- MeSH:
Adenosine Triphosphate;
Brain;
Cell Membrane;
Electrodes;
Ethanol*;
Fluorides*;
Glass;
Heart;
Hydrogen-Ion Concentration;
Permeability*;
Protons*;
Streptococcus mutans*;
Streptococcus*;
Suspensions;
Trichloroacetic Acid
- From:Journal of Korean Academy of Oral Health
2016;40(4):255-260
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: The aim of this research was to determine the pH-dependent changes in F-ATPase activity and proton fluxes in Streptococcus mutans (S. mutans) as induced by varying the concentration of fluoride ±10 mM (0.058% (v/v)) ethanol. METHODS: S. mutans UA159 was grown in Brain Heart Infusion medium at pH 4.8, 6.8, or 8.8. The F-ATPase assay was initiated by the addition of ATP, and stopped by adding 10% trichloroacetic acid. For the proton flux assay, bacterial suspensions were titrated to pH 4.6 with 0.5 M HCl, and then 0.5 M HCl was added to decrease the pH values in units of approximately 0.4 pH. The subsequent increase in pH was monitored using a glass electrode. To disrupt the cell membrane, 10% (v/v) butanol was added to the suspensions after 80 minutes. RESULTS: At all pH levels, fluoride ±10 mM ethanol not only decreased F-ATPase activity but also increased the proton permeability of S. mutans. The largest effects were observed at pH 4.8. Ethanol enhanced these effects only at pH 4.8. CONCLUSIONS: A very low concentration of ethanol enhanced the action of fluoride on F-ATPase activity and the proton permeability in S. mutans at acidic pH levels. We expect that low concentrations of ethanol may be used together with fluoride and/or other anticaries agents to develop more effective anticaries preparations.
