A Single Nucleotide Polymorphism in the E-cadherin Gene Promoter-160 is Not Associated with Risk of Korean Gastric Cancer.
10.3346/jkms.2003.18.4.501
- Author:
Won Sang PARK
1
;
Yong Gu CHO
;
Jik Young PARK
;
Chang Jae KIM
;
Jong Heun LEE
;
Hong Sug KIM
;
Jong Woo LEE
;
Young Hwa SONG
;
Cho Hyun PARK
;
Yong Kyu PARK
;
Su Young KIM
;
Suk Woo NAM
;
Sug Hyung LEE
;
Nam Jin YOO
;
Jung Young LEE
Author Information
1. Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. stingray@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cadherins;
Polymorphism;
Single Nucleotide;
Stomach Neoplasms;
Disease Susceptibility
- MeSH:
Alleles;
Cadherins/*genetics;
DNA/metabolism;
Genetic Predisposition to Disease;
Genotype;
Homozygote;
Human;
Korea;
Odds Ratio;
Polymerase Chain Reaction;
Polymorphism (Genetics);
Polymorphism, Restriction Fragment Length;
*Polymorphism, Single Nucleotide;
Polymorphism, Single-Stranded Conformational;
*Promoter Regions (Genetics);
Risk;
Stomach Neoplasms/*genetics;
Transcription, Genetic
- From:Journal of Korean Medical Science
2003;18(4):501-504
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recently, the -160 C/A polymorphism, located within the regulatory region of E-cadherin promoter, has been shown to influence E-cadherin transcription by altering transcription factor binding. We examined the effect of this polymorphism on risk of gastric cancer and on histological classification of intestinal- and diffuse-type gastric cancer in 146 normal healthy individuals and 292 Korean gastric cancer patients. Genomic DNA samples were examined by polymerase chain reaction (PCR)-single strand conformational polymorphism (SSCP)-sequencing and confirmed by restriction fragment length polymorphism (RFLP). Unexpectedly, there was no significant difference in the genotype frequencies of the polymorphism between normal control and gastric cancer patients (x(2) test, p=0.433). The estimated odd ratio of C/C to A/A genotype in gastric cancer cases was 1.07 (95% confidence interval, 0.396-2.870). We also found no evidence for differences in risk for the intestinal- and diffuse-type gastric cancer. These results suggest that the -160 C/A polymorphism of the E-cadherin has no direct effect on the risk of Korean gastric cancer development and on its histological classification.
