Tumor Necrosis Factor-alpha Gene Polymorphism (C-850T) in Korean Patients with Preeclampsia.
- Author:
Ji Hyae LIM
1
;
Shin Young KIM
;
So Yeon PARK
;
Ho Won HAN
;
Jae Hyug YANG
;
Moon Young KIM
;
Hyun Young PARK
;
Kwang Soo LEE
;
Young ju KIM
;
Hyun Mee RYU
Author Information
1. Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center, Seoul, Korea. hmryu@yahoo.com
- Publication Type:Original Article
- Keywords:
Tumor necrosis factor-alpha;
Polymorphism;
Preeclampsia
- MeSH:
Alleles;
Cytokines;
Female;
Gene Frequency;
Genotype;
Humans;
Ischemia;
Perfusion;
Pre-Eclampsia;
Pregnancy;
Promoter Regions, Genetic;
Tumor Necrosis Factor-alpha
- From:Journal of Genetic Medicine
2009;6(2):155-160
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Preeclampsia is a multisystem human pregnancy-specific disorder. The pathophysiology of preeclampsia is linked with over-stimulation of inflammatory cytokines by placental ischemia via reduced uterine perfusion pressure during pregnancy. Although an increase in tumor necrosis factor (TNF)-alpha has been reported in preeclamptic women, there is little evidence of a relationship between TNF-alpha gene variations and preeclampsia. In this study, we identified a single-nucleotide polymorphism (SNP), C-850T, in the TNF-alpha gene promoter region in Korean preeclamptic women and investigated the association between this SNP and the development of preeclampsia. MATERIALS AND METHODS: This polymorphism was analyzed in peripheral blood samples from 198 preeclamptic pregnancies and 194 normotensive pregnancies using a SNapShot kit and an ABI Prism 3100 Genetic analyzer. RESULTS: Genotypes and allele frequencies for C-850T did not differ between preeclamptic and normotensive pregnancies. The distributions of genotypes (CC, CT and TT) were 74.3%, 22.2% and 3.5%, respectively, in preeclamptic pregnancies, and 71.6%, 25.8% and 2.6%, respectively, in normotensive pregnancies. The frequencies of the C and T alleles were 0.85 and 0.15 in preeclamptic pregnancies and 0.84 and 0.16 in normotensive pregnancies, respectively. There was no increased risk of preeclampsia in subjects with the CT (OR, 0.83; P=0.44) or TT genotypes (OR, 1.32; P=0.64). CONCLUSION: We found no differences in the genotypes or allele frequencies of the TNF-alpha gene polymorphism between preeclamptic and normotensive pregnancies. This study suggests that the TNF-alpha gene polymorphism may be not associated with the development of preeclampsia in pregnant Korean women.
