Higher infiltration by Th17 cells compared with regulatory T cells is associated with severe acute T-cell-mediated graft rejection.
10.3858/emm.2011.43.11.071
- Author:
Byung Ha CHUNG
1
;
Hye Jwa OH
;
Shang Guo PIAO
;
In O SUN
;
Seok Hui KANG
;
Sun Ryoung CHOI
;
Hoon Suk PARK
;
Bum Soon CHOI
;
Yeong Jin CHOI
;
Cheol Whee PARK
;
Yong Soo KIM
;
Mi La CHO
;
Chul Woo YANG
Author Information
1. Conversant Research Consortium in Immunologic Disease, School of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. iammila@catholic.ac.kr, yangch@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
FOXP3 protein, human;
graft rejection;
interleukin-17;
Th17 cells;
T-lymphocytes, regulatory;
transplantation, homologous
- MeSH:
Acute Disease;
Creatinine/metabolism;
Forkhead Transcription Factors/metabolism;
Graft Rejection/*etiology/pathology;
Humans;
Immunoenzyme Techniques;
Interleukin-17/*metabolism;
Kidney Transplantation/*adverse effects;
Retrospective Studies;
T-Lymphocytes, Regulatory/*immunology/pathology;
Th17 Cells/*immunology/pathology;
Transplantation, Homologous
- From:Experimental & Molecular Medicine
2011;43(11):630-637
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to evaluate whether the Th17 and Treg cell infiltration into allograft tissue is associated with the severity of allograft dysfunction and tissue injury in acute T cell-mediated rejection (ATCMR). Seventy-one allograft tissues with biopsy-proven ATCMR were included. The biopsy specimens were immunostained for FOXP3 and IL-17. The allograft function was assessed at biopsy by measuring serum creatinine (Scr) concentration, and by applying the modified diet in renal disease (MDRD) formula, which provides the estimated glomerular filtration rate (eGFR). The severity of allograft tissue injury was assessed by calculating tissue injury scores using the Banff classification. The average numbers of infiltrating Treg and Th17 cells were 11.6 +/- 12.2 cells/mm2 and 5.6 +/- 8.0 cells/mm2, respectively. The average Treg/Th17 ratio was 5.6 +/- 8.2. The Treg/Th17 ratio was significantly associated with allograft function (Scr and MDRD eGFR) and with the severity of interstitial injury and tubular injury (P < 0.05, all parameters). In separate analyses of the number of infiltrating Treg and Th17 cells, Th17 cell infiltration was significantly associated with allograft function and the severity of tissue injury. By contrast, Treg cell infiltration was not significantly associated with allograft dysfunction or the severity of tissue injury. The results of this study show that higher infiltration of Th17 cell compared with Treg cell is significantly associated with the severity of allograft dysfunction and tissue injury.
