Testicular Transplantation in the Rat Model: The Comparison of Testosterone, Gonadotropin, and Histological Change in Allogenic and Isogenic Transplantation Group, and Implantation Group.
- Author:
Seong CHOI
1
;
Hyun Yul RHEW
;
Chang Hyun YOO
;
Mi Hyang KIM
;
Bang HUR
Author Information
1. Department of Urology, Kosin University, College of Medicine, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Testis;
Transplantation;
Implantation;
Gonadotropin
- MeSH:
Animals;
Aorta, Abdominal;
Follow-Up Studies;
Gonadotropins*;
Humans;
Ischemia;
Isografts;
Male;
Models, Animal*;
Organ Transplantation;
Perfusion;
Radioimmunoassay;
Rats*;
Scrotum;
Testis;
Testosterone*;
Tissue Donors;
Transplantation;
Transplants
- From:Korean Journal of Urology
1999;40(5):634-639
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The fate of avascular implants was compared with that of testicular transplants using vascular anastomosis in castrated inbred rats. MATERIALS AND METHODS: Mature inbred male rats were bilaterally castrated and received one testicle as a transplant to the scrotum. The blood supply of the grafts was reestablished by performing an end-to-side anastomosis between an aorto-caval segment of the donor bearing the testicular vessels and the abdominal aorta plus vena cava of the recipient. The average ischemia was 90 min. Lutenizing hormone(LH), follicle-stimulating hormone(FSH) and testosterone were determined by radioimmunoassay in serum samples. RESULTS: In 12 transplant recipients(6 isografts and 6 allografts), a good blood perfusion of the graft was achieved. Out of these, 6 isogenic transplants maintained normal testosterone,LH and FSH during follow up. Implant recipients,however, all displayed a strking early rise of the serum gonadotropins and concomitant castrsted levels of serum testosterone. CONCLUSIONS: Vascular transplantation rather than simple implantation is the method of choice for further research. And in allogenic testicular transplantation, there remains a problem of rejection after the operation, as in any other organ transplantation.
