IS6110-Restriction Fragment Length Polymorphism and Spoligotyping Analysis of Mycobacterium tuberculosis Clinical Isolates for Investigating Epidemiologic Distribution in Korea.
10.3346/jkms.2010.25.12.1716
- Author:
Go Eun CHOI
1
;
Mi Hee JANG
;
Eun Ju SONG
;
Seok Hoon JEONG
;
Jae Seok KIM
;
Wee Gyo LEE
;
Young UH
;
Kyoung Ho ROH
;
Hye Soo LEE
;
Jong Hee SHIN
;
Nam Hee RYOO
;
Young Ree KIM
;
Joseph JEONG
;
Jee Hee KIM
;
Sun Min LEE
;
Jongyoun YI
;
Sang Hyun HWANG
;
Hyung Hoi KIM
;
Eun Yup LEE
;
Chulhun L CHANG
;
Moon Bum KIM
;
Yeong Dae KIM
Author Information
1. Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Mycobacterium tuberculosis;
IS6110-RFLP;
Spoligotyping;
Beijing Family;
K Strain
- MeSH:
Drug Resistance, Bacterial;
Genotype;
Humans;
Microbial Sensitivity Tests;
Mycobacterium tuberculosis/*classification/genetics/isolation & purification;
Phenotype;
Polymorphism, Restriction Fragment Length;
Republic of Korea;
Tuberculosis/*epidemiology/genetics/microbiology
- From:Journal of Korean Medical Science
2010;25(12):1716-1721
- CountryRepublic of Korea
- Language:English
-
Abstract:
The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family.
