The Bacterial Protein Azurin Enhances Sensitivity of Oral Squamous Carcinoma Cells to Anticancer Drugs.
10.3349/ymj.2011.52.5.773
- Author:
Jeong Hae CHOI
1
;
Moo Hyung LEE
;
Yun Jung CHO
;
Bong Soo PARK
;
Shin KIM
;
Gyoo Cheon KIM
Author Information
1. Department of Oral Anatomy and Cell Biology, School of Dentistry, Pusan National University, Medical Research Institute, Pusan National University Hospital, Yangsan, Korea. ki91000m@pusan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Azurin;
oral squamous carcinoma;
osteosarcoma;
5-fluorouracil;
etoposide
- MeSH:
Antineoplastic Agents/*administration & dosage;
Apoptosis/drug effects;
Azurin/*administration & dosage/genetics;
Carcinoma, Squamous Cell/*drug therapy/metabolism/pathology;
Cell Line, Tumor;
Cyclin B1/metabolism;
Drug Synergism;
Etoposide/administration & dosage;
Fluorouracil/administration & dosage;
Humans;
Mouth Neoplasms/*drug therapy/metabolism/pathology;
Tumor Suppressor Protein p53/metabolism
- From:Yonsei Medical Journal
2011;52(5):773-778
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Surgical therapy is the primary treatment for oral cancer, but it can cause facial distortion. Therefore, if anticancer drugs are effective against oral cancer, they may be used preferentially. However, oral squamous carcinoma cells (OSCCs) are resistant to these drugs, so finding a way to enhance the sensitivity of these cells to anticancer drugs is important. The bacterial protein azurin is known to selectively enter cancer cells and induce apoptosis. In this study, we show the anticancer effect of azurin in OSCC. MATERIALS AND METHODS: OSCC cell line (YD-9) was subjected to azurin treatment. Cell viability, morphology and protein expression levels were monitored after treatment of azurin. Cells were also subjected to combination treatment of azurin with either 5-fluorouracil or etopside. RESULTS: Azurin-treated cells showed decreased cell viability accompanied by apoptotic phenotypes including morphological change, DNA breakage, and increases in p53 and cyclin B1 protein levels. Combination treatment of azurin with other anti-tumor agents caused an increase in sensitivity to anticancer drugs in azurin-treated YD-9 cells. CONCLUSION: Azurin has a strong synergistic anticancer effect on oral cancer cells when it is used along with anticancer drugs.
