Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR.
- Author:
Yanhua WANG
1
;
Guangxiang WANG
;
Delin ZHANG
;
Hong YIN
;
Meng WANG
Author Information
1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. wangyh061001@163.com
- Publication Type:Brief Communication ; Comparative Study ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
loop-mediated isothermal amplification (LAMP);
Q-PCR;
pig
- MeSH:
Animals;
Azure Stains;
Biological Assay;
Brain/parasitology;
DNA, Protozoan/*blood/genetics;
Lung/parasitology;
Mice;
Nucleic Acid Amplification Techniques/*veterinary;
Parasitemia;
Real-Time Polymerase Chain Reaction/veterinary;
Sensitivity and Specificity;
Swine;
Swine Diseases/*diagnosis/parasitology;
Toxoplasma/genetics/*isolation & purification;
Toxoplasmosis, Animal/*diagnosis/parasitology
- From:The Korean Journal of Parasitology
2013;51(5):573-577
- CountryRepublic of Korea
- Language:English
-
Abstract:
A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T. gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.
