Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice.
- Author:
Shin Ae KANG
1
;
Mi Kyung PARK
;
Min Kyoung CHO
;
Hak Sun YU
Author Information
1. Department of Parasitology, School of Medicine, Pusan National University, and Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Yangsan 626-870, Korea. hsyu@pusan.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Toxascaris leonina;
visceral larva migrans (VLM);
Th1;
Th2;
Th17;
cytokine
- MeSH:
Animals;
Brain/parasitology;
Cytokines/*metabolism;
Female;
Gene Expression Regulation;
Heart/parasitology;
Interleukins/*metabolism;
Intestines/parasitology;
Larva Migrans, Visceral/*immunology/parasitology;
Liver/parasitology;
Lung/parasitology/pathology;
Mice;
Mice, Inbred C57BL;
Muscles/parasitology;
Spleen/parasitology;
Th1 Cells/immunology;
Th17 Cells/immunology;
Th2 Cells/immunology;
Toxascaris/*immunology
- From:The Korean Journal of Parasitology
2013;51(5):583-588
- CountryRepublic of Korea
- Language:English
-
Abstract:
To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-alpha) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.
