Serological and Molecular Characteristics of the First Korean Case of Echinococcus multilocularis.
- Author:
Jin Sook JEONG
1
;
Sang Young HAN
;
Young Hoon KIM
;
Yasuhito SAKO
;
Tetsuya YANAGIDA
;
Akira ITO
;
Jong Yil CHAI
Author Information
1. Department of Pathology, Dong-A University College of Medicine, Busan 602-714, Korea.
- Publication Type:Brief Communication
- Keywords:
Echinococcus multilocularis;
alveolar echinococcosis;
immunodiagnosis;
cox1;
South Korea
- MeSH:
Adult;
Animals;
Antibodies, Helminth/*blood;
Antigens, Helminth/genetics/*immunology/metabolism;
Base Sequence;
Echinococcosis, Hepatic/*immunology/parasitology;
Echinococcosis, Pulmonary/diagnosis/genetics/immunology;
Echinococcus granulosus/genetics/immunology;
Echinococcus multilocularis/genetics/*immunology/isolation & purification;
Electron Transport Complex IV/genetics;
Female;
Humans;
Mitochondria/genetics;
Molecular Sequence Data;
Republic of Korea;
Sequence Analysis, DNA
- From:The Korean Journal of Parasitology
2013;51(5):595-597
- CountryRepublic of Korea
- Language:English
-
Abstract:
In December 2011, we reported an autochthonous case of Echinococcus multilocularis infection in a 42-year-old woman in Korea. The diagnosis was based on histopathological findings of the surgically resected liver cyst. In the present study, we evaluated the serological and molecular characteristics of this Korean E. multilocularis case. The patient's serum strongly reacted with affinity-purified native Em18 and recombinant Em18 antigens (specific for E. multilocularis) but negative for recombinant antigen B8/1 (reactive for Echinococcus granulosus). In immunoaffinity chromatography, the serum also strongly reacted with E. multilocularis and only weakly positive for E. granulosus. We determined the whole nucleotide sequence of cox1 (1,608 bp) using the paraffin-embedded cystic tissue which was compared with E. multilocularis isolates from China, Japan, Kazakhstan, Austria, France, and Slovakia. The Korean case showed 99.8-99.9% similarity with isolates from Asia (the highest similarity with an isolate from Sichuan, China), whereas the similarity with European isolates ranged from 99.5 to 99.6%.
