CCL4 Concentration in Tears of Dry Eye Patients and Its Correlation With Tear Surface Parameters.
10.3341/jkos.2010.51.3.313
- Author:
Seung Hyun LEE
1
;
Seoung Kyu IM
;
Kyung Chul YOON
Author Information
1. Department of Ophthalmology, Chonnam National University Medical School, Gwangju, Korea. kcyoon@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
CCL4;
Chemokine;
Dry eye syndrome;
Tear surface parameter
- MeSH:
Chemokine CCL4;
Dry Eye Syndromes;
Enzyme-Linked Immunosorbent Assay;
Eye;
Goblet Cells;
Humans;
Meibomian Glands;
Sensation;
Tears
- From:Journal of the Korean Ophthalmological Society
2010;51(3):313-319
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the expression of CCL4/MIP-1beta in tears of patients with dry eye syndrome and its correlation with the tear surface parameters. METHODS: Forty patients with dry eye syndrome (seven Meibomian gland dysfunction and 33 non-Meibomian gland dysfunction patients) and 25 control subjects were recruited. The concentrations of CCL4 in tears were measured using an enzyme-linked immunosorbent assay. The correlations between CCL4 levels and tear surface parameters including tear film break-up time (BUT), basal tear secretion (BST), tear clearance test (TCR), corneal sensation, keratoepitheliopathy (KEP) and conjunctival goblet cell density (GCD) were analyzed in patients with dry eye syndrome. RESULTS: The mean levels of MIP-1beta were 4.3+/-5.2 pg/ml in patients with dry eye syndrome and 81.6+/-294.3 pg/ml in control subjects (p=0.03). The mean levels in non-Meibomian gland disease patients (90.4+/-313.9 pg/ml) were significantly higher than those of Meibomian gland dysfunction patients (20.3+/-23.2 pg/ml, p=0.02). CCL4 levels correlated significantly with BST, TCR, KEP and GCD (p<0.05). CONCLUSIONS: The level of MIP-1beta is elevated in tears of patients with dry eye syndrome and correlates with disease severity and tear surface parameters.
