Amplification and Overexpression of c-erbB2/neu in Bladder Tumor Using Real-time Quantitative PCR with TaqMan Detection System.
- Author:
Sae Woong KIM
1
;
Ho Suk CHUNG
;
Seung Ju LEE
;
Ji Youl LEE
;
Yong Hyun CHO
;
Moon Soo YOON
;
Yong Hyun PARK
Author Information
1. Department of Urology, Catholic University Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
C-erbB2/neu gene;
Bladder cancer;
Real-time quantitative PCR
- MeSH:
Cystectomy;
Fluorescence;
Gene Amplification;
Humans;
Incidence;
Polymerase Chain Reaction*;
Recurrence;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
2001;42(9):924-933
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: C-erbB2/neu gene is potentially amplified and expressed in several tumors. In some cases, this genetic alteration seems to be a prognostic index of both overall survival and time to relapse. We investigated c-erbB2/neu gene amplification and overexpression in bladder cancer and also its relation to tumor grade, stage, and clinical outcome. MATERIALS AND METHODS: We have developed a real-time quantitative PCR assay based on TaqMan fluorescence methodology to evaluate c-erbB2/neu amplification and overexpression and prognostic significance of c-erbB2/neu overexpression in bladder cancer. The study was performed in 20 normal controls and 46 patients with bladder cancer who underwent radical cystectomy or transurethral resection. RESULTS: Twelve cases of bladder cancer (26.1%) showed c-erbB2/neu amplification and 13 cases (28.3%) showed overexpression of c-erbB2/neu. Except one case, a complete correlation between the results of amplification and overexpression of c- erbB2/neu was obtained. The incidence of c-erbB2/neu overexpression was significantly increased among the patients with high grade and/or invasive cancers (p <0.05). A strong association of c-erbB2/neu overexpression with patient survival was also found (p <0.05). CONCLUSIONS: From this study using real-time quantitative PCR, the c-erbB2/neu overexpression, together with tumor grade and stage, may be applicable as the independent prognostic factor for evaluation of invasiveness, metastatic potential and survival of bladder cancer.
