PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea.
- Author:
Yun Ah KIM
1
;
Hye Youn KIM
;
Shin Hyeong CHO
;
Hyeong Il CHEUN
;
Jae Ran YU
;
Sang Eun LEE
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Pentatrichomonas hominis; dog; phylogenetic analysis; 18S rRNA
- MeSH: Animals; Base Sequence; Cluster Analysis; DNA, Protozoan/chemistry/genetics; DNA, Ribosomal/chemistry/genetics; Diarrhea/parasitology/*veterinary; Dog Diseases/*parasitology; Dogs; Feces/*parasitology; Female; Genes, rRNA; Male; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction/methods; Protozoan Infections, Animal/*parasitology; RNA, Protozoan/genetics; RNA, Ribosomal, 18S/genetics; Republic of Korea; Sequence Analysis, DNA; Sequence Homology; Trichomonadida/*classification/genetics/*isolation & purification
- From:The Korean Journal of Parasitology 2010;48(1):9-13
- CountryRepublic of Korea
- Language:English
- Abstract: Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.
