Possible Role of Heme Oxygenase-1 and Prostaglandins in the Pathogenesis of Cerebral Malaria: Heme Oxygenase-1 Induction by Prostaglandin D2 and Metabolite by a Human Astrocyte Cell Line.
- Author:
Jiraporn KUESAP
1
;
Kesara NA-BANGCHANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Plasmodium falciparum; astrocyte; heme oxygenase; iron; cerebral malaria; prostaglandin D2
- MeSH: Animals; Astrocytes/*enzymology; Blotting, Western; Cell Line; Gene Expression Profiling; Heme Oxygenase-1/*biosynthesis; Humans; Malaria, Cerebral/*pathology; Malaria, Falciparum/*complications/*pathology; Plasmodium falciparum/*pathogenicity; Prostaglandins/*metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From:The Korean Journal of Parasitology 2010;48(1):15-21
- CountryRepublic of Korea
- Language:English
- Abstract: Astrocytes are the most abundant cells in the central nervous system that play roles in maintaining the blood-brain-barrier and in neural injury, including cerebral malaria, a severe complication of Plasmodium falciparum infection. Prostaglandin (PG) D2 is abundantly produced in the brain and regulates the sleep response. Moreover, PGD2 is a potential factor derived from P. falciparum within erythrocytes. Heme oxygenase-1 (HO-1) is catalyzing enzyme in heme breakdown process to release iron, carbon monoxide, and biliverdin/bilirubin, and may influence iron supply to the P. falciparum parasites. Here, we showed that treatment of a human astrocyte cell line, CCF-STTG1, with PGD2 significantly increased the expression levels of HO-1 mRNA by RT-PCR. Western blot analysis showed that PGD2 treatment increased the level of HO-1 protein, in a dose- and time-dependent manner. Thus, PGD2 may be involved in the pathogenesis of cerebral malaria by inducing HO-1 expression in malaria patients.
