Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats.
- Author:
Yoon Jeong CHOI
1
;
Nayoung KIM
;
Ju Yup LEE
;
Ryoung Hee NAM
;
Ji Hyung SEO
;
Seonmin LEE
;
Hee Jin KIM
;
Yoon Jin CHOI
;
Hye Seung LEE
;
Dong Ho LEE
Author Information
1. Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. nayoungkim49@empas.com
- Publication Type:Original Article
- Keywords:
S-allyl-L-cysteine;
Gastroprotection;
Anti-inflammation;
Antioxidants;
Ethanol
- MeSH:
Animals;
Antioxidants;
Blotting, Western;
Dinoprostone;
Enzyme-Linked Immunosorbent Assay;
Ethanol;
Heme Oxygenase-1;
Interleukins;
Leukotriene B4;
Mucus;
Peroxidase;
Rats*;
Rats, Sprague-Dawley;
Tumor Necrosis Factor-alpha;
Ulcer
- From:Gut and Liver
2016;10(3):348-355
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: This study aimed to examine the gastroprotective effects of PMK-S005, which is a synthetic S-allyl-L-cysteine (SAC; a sulfur-containing amino acid), against acute ethanol-induced gastric damage in rats. METHODS: Sprague-Dawley rats were divided into six groups, including a nonethanol group, groups treated with absolute ethanol 1 hour after pretreatment with various doses of PMK-S005 (1, 5, and 10 mg/kg) or rebamipide (50 mg/kg), and an absolute ethanol-only group. Ethanol-induced gross ulcer and mucus levels were measured. Myeloperoxidase, tumor necrosis factor α, interleukin 1β, PGE2, LTB4, cPLA2, COX-1, and COX-2 levels were estimated by enzyme-linked immunosorbent assay or Western blot analysis. Furthermore, the protein expression levels of antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO-1), GCLC, and GCLM, were assessed. RESULTS: PMK-S005 significantly attenuated the ethanol-induced gastric damage; it reduced mucosal inflammatory cytokine production and increased mucus levels. The expression levels of cPLA2, COX-1, and COX-2 were decreased by PMK-S005. PMK-S005 did not affect PGE2 synthesis, but LTB4 production was significantly suppressed. In addition, long-term administration of PMK-S005 significantly increased the expression of HO-1, NQO-1, GCLC, and GCLM. CONCLUSIONS: These results strongly suggest that PMK-S005 prevents gastric mucosal damage and that these gastroprotective activities are due to anti-inflammatory effects and enhancement of the gastric defense system, including antioxidant enzymes.
