Knockdown of Long Non-Coding RNA NEAT1 Inhibits Proliferation and Invasion and Induces Apoptosis of Osteosarcoma by Inhibiting miR-194 Expression.
10.3349/ymj.2017.58.6.1092
- Author:
Heping WANG
1
;
Yanzhang YU
;
Shuxin FAN
;
Leifeng LUO
Author Information
1. Department of Orthopedics, Zhoukou Central Hospital, Zhoukou, China. wangheping112@yeah.net
- Publication Type:Original Article
- Keywords:
lncRNA;
tumorigenesis;
osteosarcoma;
miR-194
- MeSH:
Apoptosis*;
Carcinogenesis;
Cell Proliferation;
Flow Cytometry;
In Vitro Techniques;
Luciferases;
Oncogenes;
Osteosarcoma*;
Real-Time Polymerase Chain Reaction;
RNA, Long Noncoding*
- From:Yonsei Medical Journal
2017;58(6):1092-1100
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogene in the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma. MATERIALS AND METHODS: Expressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively. Luciferase reporter assay was performed to observe the possible interaction between NEAT1 and miR-194. RESULTS: NEAT1 was upregulated and miR-194 was downregulated in osteosarcoma tissues and cells. Knockdown of NEAT1 or overexpression of miR-194 suppressed proliferation and invasion and induced apoptosis of osteosarcoma cells in vitro. Luciferase reporter assay validated that NEAT1 could interact with miR-194 and negatively modulated its expression. Furthermore, inhibition of miR-194 reversed the suppression of proliferation and invasion and the promotion of apoptosis induced by NEAT1 depletion in osteosarcoma cells. CONCLUSION: Knockdown of NEAT1 suppressed proliferation and invasion and induced apoptosis in osteosarcoma cells by inhibiting miR-194 expression.
