Chemotherapeutic Candidate Inducing Immunological Death of Human Tumor Cell Lines.
- Author:
Su Jin OH
1
;
Chung Kyu RYU
;
Inhak CHOI
;
So Young BAEK
;
Hyunah LEE
Author Information
- Publication Type:Brief Communication
- Keywords: Indoledione derivative; Calreticulin; Immunological death; Human tumor cells
- MeSH: Calreticulin; Cell Death; Cell Line; Cell Line, Tumor; Colon; Humans; Interleukin-12; Stomach Neoplasms
- From:Immune Network 2012;12(2):66-69
- CountryRepublic of Korea
- Language:English
- Abstract: The immunological death induction by EY-6 on the human tumor cell lines was screened. Human colon carcinoma (HCT15, HCT116), gastric carcinoma (MKN74, SNU668), and myeloma (KMS20, KMS26, KMS34) cells were died by EY-6 treatment with dose-dependent manner. CRT expression, a typical marker for the immunological death, was increased on the EY-6-treated colorectal and gastric cancer cells. Interestingly, the effects on the myeloma cell lines were complicated showing cell line dependent differential modulation. Cytokine secretion from the EY-6 treated tumor cells were dose and cell-dependent. IFN-gamma and IL-12 secretion was increased in the treated cells (200% to over 1000% of non-treated control), except HCT116, SNU668 and KMS26 cells which their secretion was declined by EY-6. Data suggest the potential of EY-6 as a new type of immuno-chemotherapeutics inducing tumor-specific cell death. Further studies are planned to confirm the efficacy of EY-6 including in vivo study.
