Muscle Derived Stem Cell/Alginate/Polycaprolactone/ Injection Therapy in Rats with Denervated Urethral Sphincter.
10.4111/kju.2007.48.12.1296
- Author:
Haet Nim LEE
1
;
Ji Young LEE
;
Jun Sung KOH
;
Hyun Woo KIM
;
Seok Soo BYUN
;
Sang Seob LEE
;
Ji Youl LEE
Author Information
1. Department of Urology, College of Medicine, The Catholic University of Korea, Korea. uroljy@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Muscle derived stem cell;
Alginate;
Polycaprolactone;
Urinary incontinence
- MeSH:
Animals;
Denervation;
Female;
Humans;
Models, Animal;
Muscle, Skeletal;
Pudendal Nerve;
Rats*;
Stem Cells;
Urethra*;
Urinary Incontinence
- From:Korean Journal of Urology
2007;48(12):1296-1301
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In this study, we tested whether injections of muscle-derived stem cells and alginate(Alg)/polycaprolactone(PCL) after denervation of the pudendal nerve could increase the leak point pressure(LPP) and closing pressure(CP) over the long term in a rat model of urinary incontinence. MATERIALS AND METHODS: Muscle derived stem cells(MDSC) were isolated from the gastrocnemius muscle of normal female rats, and these cells were purified for creating a myogenic population by the preplate technique. In the denervated(D) group, the pudendal nerve was transected bilaterally via a dorsal incision in order to denervate the external urethral sphincter. The denervated external urethral sphincter was injected with Alg/PCL(AP group), or MDSC/Alg/PCL(M group) into the proximal urethra after pudendal nerve transection. At 1 and 3 months, the LPP and CP measurements were visually identified by using the vertical tilt/intravesical pressure clamp model of stress urinary incontinence. The rats were then sacrificed and their urethras were harvested for histology. RESULTS: Both the LPP and CP were significantly lower in the denervated group at each time compared with the normal(N group), AP and M groups, and both the LPP and CP in the N, AP and M groups were significantly higher than those in the D group at both 1 and 3 months. The persistence of MDSC over the period of the study was verified by histology. Thus, pudendal nerve denervation led to a progressive decline in the LPP and CP that was evident at 1 month and this persisted to 3 months, and injection of MDSC/Alg/PCL into the denervated rats led to a long term increase in the LPP and CP. CONCLUSIONS: The N, AP and M groups all had significantly higher LPPs than the D group, and MDSC/Alg/PCL injection into the denervated external urethral sphincter in female rats increased the LPP and CP in both the short and long term. We also observed a long term bulking effect of MDSC/Alg/PCL injection in the stress incontinence animal model.
