Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules.
10.3803/EnM.2016.31.4.586
- Author:
Hyemi KWON
1
;
Won Gu KIM
;
Markus ESZLINGER
;
Ralf PASCHKE
;
Dong Eun SONG
;
Mijin KIM
;
Suyeon PARK
;
Min Ji JEON
;
Tae Yong KIM
;
Young Kee SHONG
;
Won Bae KIM
Author Information
1. Division of Endocrinology and Metabolism, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. kimwb@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Molecular diagnostic techniques;
Biopsy, fine-needle;
Thyroid aspiration;
Thyroid nodule;
Thyroid neoplasms
- MeSH:
Biopsy, Fine-Needle;
Diagnosis*;
DNA;
Humans;
Molecular Diagnostic Techniques;
Point Mutation;
Prospective Studies;
Real-Time Polymerase Chain Reaction;
RNA;
Thyroid Gland*;
Thyroid Neoplasms;
Thyroid Nodule*
- From:Endocrinology and Metabolism
2016;31(4):586-591
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Molecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis. METHODS: This prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAF(V600E) and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing. RESULTS: All DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality. CONCLUSION: Molecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules.
