A Study of Surface Physical Properties of New Surfactant Using Synthetic Peptides of Surfactant Protein-B.
- Author:
Chong Woo BAE
1
;
Yong Mook CHOI
;
Joo Hyun KANG
;
Sang Chul LEE
;
Kil Lyong KIM
;
Kyung Soo HAHM
Author Information
1. Department of Pediatrics, College of Medicine, Kyunghee University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Pulmonary surfactant;
Surfactant protein(SP);
SP-B;
Peptides;
Respiratory distress syndrome(RDS)
- MeSH:
Absorption;
Adsorption;
Amino Acid Sequence;
Humans;
Peptides*;
Pulmonary Surfactants
- From:Journal of the Korean Pediatric Society
2000;43(8):1059-1067
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To produce a new generation of artificial pulmonary surfactant(PS), surfactant protein (SP)-B from human PSwas isolated, and the amino acid sequences of these proteins were studied. Artificial peptides of human SP-B were synthesized. New artificial PS preparations which were cornposed of phospholopids and two artificial synthetic SP-B peptides were made, and the surface physical properties of these new PS preparations were tested. METHODS: The purities of SP-B were assessed by SDS-polyacrylamide gel and the amino acid sequences of these proteins were determined. We synthetized two peptides SP-1 and SP-2 and the amino acid sequences were as follows,' SP-1: RMLPQLVCRLVLRCSMD, SP-2: RMLP- QLVCRLVLRCSM. Surface physical properties of newly artificial PSs, which were composed of a mixture of phospholipid(PL) and SP-1 or SP-2(sample A; PL+SP-1, sample B; PL+SP-2), were measured by surface spreading, adsorption rate, and surface tension-area diagram. RESULTS: The amino acid sequence of human SP-B was obtained. We produced the artificial peptides of SP-B and prepared the new generation PS(sample A and sample B). The order of the superiority of spreading and adsorption rate was Surfacten
