Relationship of the Changes in Biogenic Amines to Nitric Oxide and Oxygen Free Radicals During Cerebral Ischemia/Reperfusion.
- Author:
Joon Shik MOON
1
;
Hee Sun JUNG
;
Dong Goo KIM
;
Kyung Hwan KIM
;
Byung Chul LEE
Author Information
1. Department of Neurology, Yonsei University College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Ischemia;
Reperfusion;
Biogenic amine;
Dopamine;
DMTU;
NMMA
- MeSH:
3,4-Dihydroxyphenylacetic Acid;
Anoxia;
Biogenic Amines*;
Carotid Artery, Common;
Corpus Striatum;
Dopamine;
Free Radicals*;
Gerbillinae;
Ischemia;
Neurons;
Nitric Oxide*;
omega-N-Methylarginine;
Oxygen*;
Reperfusion;
Reperfusion Injury
- From:Journal of the Korean Neurological Association
1995;13(4):773-787
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Recently oxygen free radicals and nitric oxide (NO) are known to play an important role in neuronal reperfusion injury. This study was aimed to investigate the role of oxygen f ree radicals and NO during cerebral ischemia/reperfusion, using dimethylthiourea (DMTU) and NG-monomethyl-L-arginine (NMMA), an oxygen f ree radical scavenger and a competitive NOS inhibitor respectively. In the in vivo experiment, the ischemia/reperfusion-induced changes of cerebral biogenic amines were examined in Mongolian gerbil (Meriones unguiculatus) pre-treated with NMMA and/or DMTU. To induce cerebral ischemia/reperfusion, bilateral common carotid arteries were clamped for 10 minutes and then released for 15 minutes. The biogenic amines were measured by using HPLC-ECD(High Performance Liquid Chromatography-Electrochemical detection). To confirm the results from the in vivo experiments, the effect of NMMA and/or DMTU on [3H]dopamine release from striatal slices exposed to hypoxia was investigated. The results are as follows; 1) Ischemia/reperfusion increased the ratio of DOPAC/dopamine and HVA/dopamine as well as the concentrations of DOPAC and HVA, which were evident only in corpus striatum. 2) NMMA attenuated the ischemia/reperfusion-induced increase in the ratio of DOPAC/dopamine in corpus striatum. However, the change of DOPAC or HVA was minimal. 3) DMTU attenuated the ischemia/reperfusion-induced increase of DOPAC and HVA, and the ratio ofDOPAC / dopa- mine and HVA/dopamine in corpus striatum. 4) Simultaneous pre-treatment with NMMA and DMTU attenuated the ischemia/reperfusion-induced increase of DOPAC and HVA, and the ratio Of DOPAC/dopamine and HVA/dopamine in corpus striatum. The extent of attenuation was greater than the single treatment group with NMMA or DMTU. 5) Exposure to hypoxia markedly increased the release of [3H]dopamine in the striatal slices. 6) The administration of either NMMA or DMTU attenuated the increase of [3H]dopamine release induced by hypoxia in the striatal slices. 7) The administration of both NMMA or DMTU markedly attenuated the increase of [3H]dopamine release induced by hypoxia to the extent of the control in the striatal slices. These results suggest that oxygen free radicals play an important role in cerebral ischemia/reperfusion injury, for which NO seems to be responsible.
