Development and Evaluation of Indirect ELISA for Detection of Antibodies to Getah Virus in Horse Serum.
- Author:
Seung Heon LEE
1
;
Dong Kun YANG
;
Ha Hyun KIM
;
Hyun Ye JO
;
Sung Suk CHOI
;
In Soo CHO
Author Information
- Publication Type:Original Article
- Keywords: Getah virus; ELISA; Racehorse
- MeSH: Alphavirus*; Antibodies*; Enzyme-Linked Immunosorbent Assay*; Horses*; Humans; Korea; Mass Screening; Sensitivity and Specificity; Swine; Togaviridae; Vertebrates
- From:Journal of Bacteriology and Virology 2016;46(2):63-70
- CountryRepublic of Korea
- Language:English
- Abstract: Getah virus (GETV) is a member of the genus Alphavirus in the family Togaviridae. GETV infection can occur in a wide range of vertebrate species, and the virus has been known for a pathogen of horses and pigs. To rapidly and accurately diagnose GETV infection of a racehorse, an indirect ELISA (I-ELISA) was developed in the present study for detection of antibodies to GETV in serum samples. To evaluate the developed I-ELISA, a total of 240 serum samples from Thoroughbred racehorses raised in Korea were screened in parallel by a serum neutralization (SN) test. The developed I-ELISA exhibited an efficacy comparable to that of the SN test in terms of a high diagnostic sensitivity (86.3%) and specificity (94.5%) at a cut-off absorbance value of 0.25. In addition, our results showed that the developed I-ELISA had a significant correlation with the SN test (r = 0.91; p < 0.05). Taken together, our findings suggest that the I-ELISA developed in this study is a valuable diagnostic tool for the screening of horses suspected to be infected with GETV.
