The Diagnostic Value of Polymerase Chain Reaction in Intestinal Tuberculosis.
- Author:
Tae Kyu LEE
1
;
Young Hwan KIM
;
U Im CHANG
;
Eun Jung JUN
;
Jin Il KIM
;
Soo Heon PARK
;
Joon Yeol HAN
;
Jae Kwang KIM
;
Kyu Won CHUNG
;
Hee Sik SUN
Author Information
1. Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.jkkim@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Intestinal tuberculosis;
Polymerase chain reaction (PCR)
- MeSH:
Crohn Disease;
Diagnosis, Differential;
DNA;
Granuloma;
Meningitis;
Mycobacterium tuberculosis;
Pleurisy;
Polymerase Chain Reaction*;
Tuberculosis*;
Tuberculosis, Pulmonary
- From:Korean Journal of Gastrointestinal Endoscopy
2003;26(2):79-83
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND/AIMS: It is quite difficult to differentiate intestinal tuberculosis from Crohn's disease because of the similarities of their clinical and pathological features and low detection rate of acid fast bacilli. The diagnostic value of PCR has been studied in pulmonary tuberculosis, tuberculous pleuritis and meningitis, but few reports were made in cases of intestinal tuberculosis. The aim of this study is to evaluate the diagnostic value of PCR in intestinal tuberculosis. METHODS: The subjects, a total of 70 cases are composed of clinically diagnosed intestinal tuberculosis, Crohn's disease and intestinal Behcet's disease. We performed PCR with paraffin-embedded intestinal tissue to detect the DNA of Mycobacterium Tuberculosis and the data was analyzed. RESULTS: The positive rate of PCR for Mycobacterium Tuberculosis was 9.8% (4/41) in intestinal tuberculosis, 0% (0/29) in Crohn's disease, and 0% (0/3) in intestinal Behcet's disease. The granulomas were not found in 50% (2/4) of the PCR positive intestinal tuberculosis cases. CONCLUSIONS: We were not able to find evidences to prove the excellent value of PCR assay in making differential diagnosis of intestinal tuberculosis from other granulomatous diseases. But it might be helpful in detecting cases of intestinal tuberculosis which were not pathologically or microbiologically confirmed.
