Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection.
10.3349/ymj.2008.49.5.828
- Author:
Byungki CHO
1
;
Bo Young JEON
;
Jungho KIM
;
Jaesang NOH
;
Jiha KIM
;
Minjung PARK
;
Sun PARK
Author Information
1. Standard Diagnostics Inc., Yongin, Korea. bojeon@yuhs.ac
- Publication Type:Original Article ; Evaluation Studies ; Research Support, Non-U.S. Gov't
- Keywords:
Chikungunya virus;
E1 and E2;
envelope protein;
baculovirus;
indirect IgM ELISA
- MeSH:
Alphavirus Infections/*diagnosis;
Animals;
Baculoviridae/genetics/metabolism;
Cells, Cultured;
Chikungunya virus/genetics;
Cloning, Molecular;
Enzyme-Linked Immunosorbent Assay/methods;
Recombinant Proteins/immunology;
Sensitivity and Specificity;
Serologic Tests/*methods;
Viral Envelope Proteins/*immunology
- From:Yonsei Medical Journal
2008;49(5):828-835
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens. MATERIALS AND METHODS: CHIKV E1, and E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 and E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA. RESULTS: The recombinant CHIKV E1 and E2 envelope protein showed sensitivity of 77.5% and 90%, respectively. The specificities of both CHIKV E1 and E2 envelope proteins were 100%. CONCLUSION: The recombinant CHIKV E1 and E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection.
