Localization of Intracellular IVIonoclonal Antibody Specific for IVlycobacteria in Experimentally Induced Pulmonary Tuberculosis.
10.3348/jkrs.1994.31.2.267
- Author:
Sang Nae CHO
;
Dong Soo KIM
;
Dong Hwan SHIN
;
Jeon Soo SHIN
;
Se Jong KIM
;
Chang Yun PARK
;
Jong Doo LEE
;
Soon Hee CHUNG
- Publication Type:Original Article
- MeSH:
Animals;
Biotin;
Cytoplasm;
Guinea Pigs;
Humans;
Immunoglobulin G;
Mice;
Monocytes;
Mycobacterium tuberculosis;
Rabbits;
Streptavidin;
Tuberculosis;
Tuberculosis, Pulmonary*
- From:Journal of the Korean Radiological Society
1994;31(2):267-271
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To determine whether intracellular localization of intravenously injected infection specific MoAb can be localized in the infected cells, immunohistochemical staining was obtained in animals infected with Mycobacterium tuberculosis. MATERIALS AND METHODS: One mg of intact mouse MoAb against mwobacteria (group I, n=10) and F(ab')2 (group II, n=6) were intravenously injected to the rabbits of each groups infected with M. tuberculosis H37RV. Immunohistochemical staining using an anti-mouse antibody was obtained at the 1,3, 5, 7 and 8th days in Group I and at the 1, 2, 3rd days in group II by streptavidin biotin method. For the control study, I mg of non specific polyclonal human IgG (group III, n=10) and 100 microgram of normal rabbit IgG F(ab')2 (group IV, n=6) were injected to the rabbits and guinea pigs respectively. RESULTS: Both groups (group I & II) showed a positive Ag-Ab reaction within the cytoplasm of monocytes. A weak but positive reaction was observed intracellularly in group III, however no positive reaction in group IV. CONCLUSION: Our results suggest intracellular Ag-Ab reaction plays an important role in the localization of infection by immunoscintigraphy using a specific MoAb fragments.
